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type vii collagen antibody  (Millipore)


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    Millipore type vii collagen antibody
    a , qRT-PCR analysis of HRH1 , MMP2 , and MMP9 in skin organoids at day 7, upon histamine or antihistamine and histamine treatment. Hist-histamine, CF-clemastine fumarate. Results are representative of independent experiments conducted in replicates. Two-tailed Student’s t-tests calculated the P-value by comparing Hist to control, then CF to the Hist group for each gene. Error bar-standard deviation. b , Keratin 5 (red) and type <t>VII</t> <t>collagen</t> (green) immunofluorescence of oncogenic HRAS and CDK4 -transformed human skin organoids following treatment with histamine or the P38 inhibitor SB203580. Scale bar=100μm, conducted with replicates and repeated three independent times.
    Type Vii Collagen Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/type+vii+collagen+antibody/type+vii+collagen+antibody/bio_rxiv__2025__06__23__661147-301-9-14
    Average 90 stars, based on 1 article reviews
    type vii collagen antibody - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Epithelial tumor cells utilize mast cell-derived histamine to regulate perineural invasion"

    Article Title: Epithelial tumor cells utilize mast cell-derived histamine to regulate perineural invasion

    Journal: bioRxiv

    doi: 10.1101/2025.06.23.661147

    a , qRT-PCR analysis of HRH1 , MMP2 , and MMP9 in skin organoids at day 7, upon histamine or antihistamine and histamine treatment. Hist-histamine, CF-clemastine fumarate. Results are representative of independent experiments conducted in replicates. Two-tailed Student’s t-tests calculated the P-value by comparing Hist to control, then CF to the Hist group for each gene. Error bar-standard deviation. b , Keratin 5 (red) and type VII collagen (green) immunofluorescence of oncogenic HRAS and CDK4 -transformed human skin organoids following treatment with histamine or the P38 inhibitor SB203580. Scale bar=100μm, conducted with replicates and repeated three independent times.
    Figure Legend Snippet: a , qRT-PCR analysis of HRH1 , MMP2 , and MMP9 in skin organoids at day 7, upon histamine or antihistamine and histamine treatment. Hist-histamine, CF-clemastine fumarate. Results are representative of independent experiments conducted in replicates. Two-tailed Student’s t-tests calculated the P-value by comparing Hist to control, then CF to the Hist group for each gene. Error bar-standard deviation. b , Keratin 5 (red) and type VII collagen (green) immunofluorescence of oncogenic HRAS and CDK4 -transformed human skin organoids following treatment with histamine or the P38 inhibitor SB203580. Scale bar=100μm, conducted with replicates and repeated three independent times.

    Techniques Used: Quantitative RT-PCR, Two Tailed Test, Control, Standard Deviation, Immunofluorescence, Transformation Assay

    Related Articles

    Staining:

    Article Title: In vivo and in vitro approaches in understanding the differences between Caucasian and African skin types: specific involvement of the papillary dermis.
    Article Snippet: .. Mouse monoclonal primary antibodies were used to stain human type IV collagen (Dako, Glostrop, Denmark), type VII collagen (Chemicon, Millipore, Billerica, MA, USA), laminin 5 (Chemicon) proteins, and rabbit polyclonal primary antibody for human nidogen protein (Calbiochem, Nottingham, UK). .. Primary antibodies were revealed by fluorescein isothiocyanate-conjugated rabbit antimouse or swine antirabbit antibodies (Dako).



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    a , qRT-PCR analysis of HRH1 , MMP2 , and MMP9 in skin organoids at day 7, upon histamine or antihistamine and histamine treatment. Hist-histamine, CF-clemastine fumarate. Results are representative of independent experiments conducted in replicates. Two-tailed Student’s t-tests calculated the P-value by comparing Hist to control, then CF to the Hist group for each gene. Error bar-standard deviation. b , Keratin 5 (red) and type <t>VII</t> <t>collagen</t> (green) immunofluorescence of oncogenic HRAS and CDK4 -transformed human skin organoids following treatment with histamine or the P38 inhibitor SB203580. Scale bar=100μm, conducted with replicates and repeated three independent times.
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    Image Search Results


    a , qRT-PCR analysis of HRH1 , MMP2 , and MMP9 in skin organoids at day 7, upon histamine or antihistamine and histamine treatment. Hist-histamine, CF-clemastine fumarate. Results are representative of independent experiments conducted in replicates. Two-tailed Student’s t-tests calculated the P-value by comparing Hist to control, then CF to the Hist group for each gene. Error bar-standard deviation. b , Keratin 5 (red) and type VII collagen (green) immunofluorescence of oncogenic HRAS and CDK4 -transformed human skin organoids following treatment with histamine or the P38 inhibitor SB203580. Scale bar=100μm, conducted with replicates and repeated three independent times.

    Journal: bioRxiv

    Article Title: Epithelial tumor cells utilize mast cell-derived histamine to regulate perineural invasion

    doi: 10.1101/2025.06.23.661147

    Figure Lengend Snippet: a , qRT-PCR analysis of HRH1 , MMP2 , and MMP9 in skin organoids at day 7, upon histamine or antihistamine and histamine treatment. Hist-histamine, CF-clemastine fumarate. Results are representative of independent experiments conducted in replicates. Two-tailed Student’s t-tests calculated the P-value by comparing Hist to control, then CF to the Hist group for each gene. Error bar-standard deviation. b , Keratin 5 (red) and type VII collagen (green) immunofluorescence of oncogenic HRAS and CDK4 -transformed human skin organoids following treatment with histamine or the P38 inhibitor SB203580. Scale bar=100μm, conducted with replicates and repeated three independent times.

    Article Snippet: Primary antibodies used were keratin 5 (rabbit, 1:500, BioLegend), type VII collagen (mouse, 1:250, Millipore Sigma), and type IV collagen (rabbit, 1:100, Abcam).

    Techniques: Quantitative RT-PCR, Two Tailed Test, Control, Standard Deviation, Immunofluorescence, Transformation Assay

    Identification of COL7A1 as a BM-related key biomarker in LUAD. (A) Univariate Cox regression analysis of 12 candidate genes. (B–D) The expression levels of three prognosis-associated genes, including COL7A1 (B) , MMP1 (C) , and LAMA3 (D) , in LUAD samples and controls of the TCGA dataset (*p < 0.05, **p < 0.01, ***p < 0.001).

    Journal: Frontiers in Pharmacology

    Article Title: COL7A1 indicates crucial potential as a basal membrane-related prognostic biomarker and therapeutic target in lung adenocarcinoma

    doi: 10.3389/fphar.2025.1543193

    Figure Lengend Snippet: Identification of COL7A1 as a BM-related key biomarker in LUAD. (A) Univariate Cox regression analysis of 12 candidate genes. (B–D) The expression levels of three prognosis-associated genes, including COL7A1 (B) , MMP1 (C) , and LAMA3 (D) , in LUAD samples and controls of the TCGA dataset (*p < 0.05, **p < 0.01, ***p < 0.001).

    Article Snippet: In this experiment, the primary antibody was COL7A1 Polyclonal Antibody (19799-1-AP, Proteintech Group, Wuhan, China) and GAPDH Antibody (60004-1-Ig, Proteintech Group, Wuhan, China), and the secondary antibody was horseradase labeling of goat with anti-rabbit IgG 1:1,000 (ZB-2301-0.1mL, Beijing Zhong Shan-Golden Bridge Biological Technology Co., Ltd., Beijing, China) and horseradase labeling of goat anti-mouse IgG 1:1,000 (ZB-2301-0.1mL, Beijing Zhong Shan-Golden Bridge Biological Technology Co., Ltd., Beijing, China).

    Techniques: Biomarker Assay, Expressing

    Validation of the COL7A1 expression levels. (A–C) Box plot of COL7A1 expression in LUAD and controls of GSE115002 (A) , GSE11072 (B) cohorts, and CCLE database (C) . (D) Immunohistochemical plots of COL7A1 in lung tissue downloaded from the HPA database, with LUAD tissue on the left and normal lung tissue on the right. (E, F) Expression of COL7A1 in LUAD determined by qPCR (E) and Western blot (F) . (*p < 0.05, **p < 0.01, ***p < 0.001).

    Journal: Frontiers in Pharmacology

    Article Title: COL7A1 indicates crucial potential as a basal membrane-related prognostic biomarker and therapeutic target in lung adenocarcinoma

    doi: 10.3389/fphar.2025.1543193

    Figure Lengend Snippet: Validation of the COL7A1 expression levels. (A–C) Box plot of COL7A1 expression in LUAD and controls of GSE115002 (A) , GSE11072 (B) cohorts, and CCLE database (C) . (D) Immunohistochemical plots of COL7A1 in lung tissue downloaded from the HPA database, with LUAD tissue on the left and normal lung tissue on the right. (E, F) Expression of COL7A1 in LUAD determined by qPCR (E) and Western blot (F) . (*p < 0.05, **p < 0.01, ***p < 0.001).

    Article Snippet: In this experiment, the primary antibody was COL7A1 Polyclonal Antibody (19799-1-AP, Proteintech Group, Wuhan, China) and GAPDH Antibody (60004-1-Ig, Proteintech Group, Wuhan, China), and the secondary antibody was horseradase labeling of goat with anti-rabbit IgG 1:1,000 (ZB-2301-0.1mL, Beijing Zhong Shan-Golden Bridge Biological Technology Co., Ltd., Beijing, China) and horseradase labeling of goat anti-mouse IgG 1:1,000 (ZB-2301-0.1mL, Beijing Zhong Shan-Golden Bridge Biological Technology Co., Ltd., Beijing, China).

    Techniques: Expressing, Immunohistochemical staining, Western Blot

    Exploration of the association of COL7A1 expression with prognosis of LUAD patients. (A–C) The Kaplan-Meier curves of COL7A1 high expression and COL7A1 low expression groups in the LUAD patients of TCGA (A) , GSE72094 (B) , and GSE68465 (C) datasets. (D–F) Time-dependent ROC curves of the COL7A1 expression for predicting 1-, 3-, and 5-year survival in the TCGA (D) , GSE72094 (E) , and GSE68465 (F) datasets. (G) Univariate and multivariate Cox regression analysis of COL7A1 expression and clinical features including age, gender, clinical stage, and race.

    Journal: Frontiers in Pharmacology

    Article Title: COL7A1 indicates crucial potential as a basal membrane-related prognostic biomarker and therapeutic target in lung adenocarcinoma

    doi: 10.3389/fphar.2025.1543193

    Figure Lengend Snippet: Exploration of the association of COL7A1 expression with prognosis of LUAD patients. (A–C) The Kaplan-Meier curves of COL7A1 high expression and COL7A1 low expression groups in the LUAD patients of TCGA (A) , GSE72094 (B) , and GSE68465 (C) datasets. (D–F) Time-dependent ROC curves of the COL7A1 expression for predicting 1-, 3-, and 5-year survival in the TCGA (D) , GSE72094 (E) , and GSE68465 (F) datasets. (G) Univariate and multivariate Cox regression analysis of COL7A1 expression and clinical features including age, gender, clinical stage, and race.

    Article Snippet: In this experiment, the primary antibody was COL7A1 Polyclonal Antibody (19799-1-AP, Proteintech Group, Wuhan, China) and GAPDH Antibody (60004-1-Ig, Proteintech Group, Wuhan, China), and the secondary antibody was horseradase labeling of goat with anti-rabbit IgG 1:1,000 (ZB-2301-0.1mL, Beijing Zhong Shan-Golden Bridge Biological Technology Co., Ltd., Beijing, China) and horseradase labeling of goat anti-mouse IgG 1:1,000 (ZB-2301-0.1mL, Beijing Zhong Shan-Golden Bridge Biological Technology Co., Ltd., Beijing, China).

    Techniques: Expressing

    Exploration of the biological significance of different COL7A1 expression groups. (A) The top 10 KEGG pathways with significant differences between the high COL7A1 expression and low COL7A1 expression groups. (B) Five important KEGG pathways were activated in the high COL7A1 expression group based on GSEA. (C) The top 5 GO terms with significant differences between the high COL7A1 expression and low COL7A1 expression groups. (D) Five important GO terms were activated in the high COL7A1 expression group based on GSEA.

    Journal: Frontiers in Pharmacology

    Article Title: COL7A1 indicates crucial potential as a basal membrane-related prognostic biomarker and therapeutic target in lung adenocarcinoma

    doi: 10.3389/fphar.2025.1543193

    Figure Lengend Snippet: Exploration of the biological significance of different COL7A1 expression groups. (A) The top 10 KEGG pathways with significant differences between the high COL7A1 expression and low COL7A1 expression groups. (B) Five important KEGG pathways were activated in the high COL7A1 expression group based on GSEA. (C) The top 5 GO terms with significant differences between the high COL7A1 expression and low COL7A1 expression groups. (D) Five important GO terms were activated in the high COL7A1 expression group based on GSEA.

    Article Snippet: In this experiment, the primary antibody was COL7A1 Polyclonal Antibody (19799-1-AP, Proteintech Group, Wuhan, China) and GAPDH Antibody (60004-1-Ig, Proteintech Group, Wuhan, China), and the secondary antibody was horseradase labeling of goat with anti-rabbit IgG 1:1,000 (ZB-2301-0.1mL, Beijing Zhong Shan-Golden Bridge Biological Technology Co., Ltd., Beijing, China) and horseradase labeling of goat anti-mouse IgG 1:1,000 (ZB-2301-0.1mL, Beijing Zhong Shan-Golden Bridge Biological Technology Co., Ltd., Beijing, China).

    Techniques: Expressing

    The landscape of immune cell infiltration between different groups. (A–D) Box plot of tumor purity (A) , immune (B) , stromal (C) , and ESTIMATE (D) scores in different COL7A1 expression groups based on the GSE68465 dataset. (E, F) TStacked plot of 22 immune cell types in each sample of TCGA-LUAD (E) and GSE68465 (F) cohorts. (G, H) The 22 immune cell infiltration in the high COL7A1 expression and low COL7A1 expression groups of TCGA-LUAD (G) and GSE68465 (H) cohorts. (*p < 0.05, **p < 0.01, ***p < 0.001)

    Journal: Frontiers in Pharmacology

    Article Title: COL7A1 indicates crucial potential as a basal membrane-related prognostic biomarker and therapeutic target in lung adenocarcinoma

    doi: 10.3389/fphar.2025.1543193

    Figure Lengend Snippet: The landscape of immune cell infiltration between different groups. (A–D) Box plot of tumor purity (A) , immune (B) , stromal (C) , and ESTIMATE (D) scores in different COL7A1 expression groups based on the GSE68465 dataset. (E, F) TStacked plot of 22 immune cell types in each sample of TCGA-LUAD (E) and GSE68465 (F) cohorts. (G, H) The 22 immune cell infiltration in the high COL7A1 expression and low COL7A1 expression groups of TCGA-LUAD (G) and GSE68465 (H) cohorts. (*p < 0.05, **p < 0.01, ***p < 0.001)

    Article Snippet: In this experiment, the primary antibody was COL7A1 Polyclonal Antibody (19799-1-AP, Proteintech Group, Wuhan, China) and GAPDH Antibody (60004-1-Ig, Proteintech Group, Wuhan, China), and the secondary antibody was horseradase labeling of goat with anti-rabbit IgG 1:1,000 (ZB-2301-0.1mL, Beijing Zhong Shan-Golden Bridge Biological Technology Co., Ltd., Beijing, China) and horseradase labeling of goat anti-mouse IgG 1:1,000 (ZB-2301-0.1mL, Beijing Zhong Shan-Golden Bridge Biological Technology Co., Ltd., Beijing, China).

    Techniques: Expressing

    Validation of COL7A1 expression in predicting the clinical benefit of immunotherapy. (A) IC50 values of the 11 drugs in the COL7A1-H and COL7A1-L groups. (B) The expression levels of immune checkpoint genes in different COL7A1 expression groups. (C) . Correlation analysis of immune checkpoint genes and COL7A1 expression (the data conformed to normal distribution, employing pearson correlation analysis) (D–I) Violin plots of TIDE (D) , Exclusion (E) , Dysfunction (F) , MDSC (G) , CAF (H) , and CD274 (I) scores in different COL7A1 expression groups.

    Journal: Frontiers in Pharmacology

    Article Title: COL7A1 indicates crucial potential as a basal membrane-related prognostic biomarker and therapeutic target in lung adenocarcinoma

    doi: 10.3389/fphar.2025.1543193

    Figure Lengend Snippet: Validation of COL7A1 expression in predicting the clinical benefit of immunotherapy. (A) IC50 values of the 11 drugs in the COL7A1-H and COL7A1-L groups. (B) The expression levels of immune checkpoint genes in different COL7A1 expression groups. (C) . Correlation analysis of immune checkpoint genes and COL7A1 expression (the data conformed to normal distribution, employing pearson correlation analysis) (D–I) Violin plots of TIDE (D) , Exclusion (E) , Dysfunction (F) , MDSC (G) , CAF (H) , and CD274 (I) scores in different COL7A1 expression groups.

    Article Snippet: In this experiment, the primary antibody was COL7A1 Polyclonal Antibody (19799-1-AP, Proteintech Group, Wuhan, China) and GAPDH Antibody (60004-1-Ig, Proteintech Group, Wuhan, China), and the secondary antibody was horseradase labeling of goat with anti-rabbit IgG 1:1,000 (ZB-2301-0.1mL, Beijing Zhong Shan-Golden Bridge Biological Technology Co., Ltd., Beijing, China) and horseradase labeling of goat anti-mouse IgG 1:1,000 (ZB-2301-0.1mL, Beijing Zhong Shan-Golden Bridge Biological Technology Co., Ltd., Beijing, China).

    Techniques: Expressing